Basic Vector Information
- Vector Name:
- MSGV-1D3-28Z-Puro-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9613 bp
- Type:
- Protein expression, Antibody expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Retrovirus
- Selection Marker:
- Puro
- Promoter:
- MSCV
- 5' Primer:
- MSGV-1D3-28Z All ITAMs intact
- 3' Primer:
- pEGFP-C-5'
- Growth Temperature:
- 37℃
MSGV-1D3-28Z-Puro-EGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
MSGV-1D3-28Z-Puro-EGFP vector Sequence
LOCUS 62056_1680 9613 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9613) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..9613 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 1..517 /label=5' LTR /note="5' long terminal repeat from murine embryonic stem cell virus" CDS 1020..1427 /codon_start=1 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" /translation="VTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTFNVG WPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKPPPP LPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA" misc_feature 1437..1807 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" promoter 3272..3771 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" CDS 3798..4514 /codon_start=1 /label=EGFP /note="enhanced GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" misc_feature 5283..5871 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 5998..6512 /label=3' LTR /note="3' long terminal repeat from murine embryonic stem cell virus" protein_bind complement(6684..6700) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6708..6738) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(6753..6774) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7062..7650) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7824..8681) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(8682..8786) /label=AmpR promoter
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