Basic Vector Information
- Vector Name:
- pLV2-TRE3GS-EGFP-TetOne-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9932 bp
- Type:
- Protein expression, Tetracycline inducible
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Puro
- Promoter:
- hPGK
- Growth Temperature:
- 37℃
pLV2-TRE3GS-EGFP-TetOne-Puro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV2-TRE3GS-EGFP-TetOne-Puro vector Sequence
LOCUS V015090 9932 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V015090
VERSION V015090
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9932)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..9932
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal complement(216..350)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 398..502
/label="AmpR promoter"
CDS 503..1360
/label="AmpR"
/note="beta-lactamase"
rep_origin 1534..2119
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2407..2428
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2443..2473
/label="lac promoter"
/note="promoter for the E. coli lac operon"
LTR complement(2650..3283)
/label="5' LTR"
/note="5' long terminal repeat (LTR) from HIV-1"
misc_feature complement(3490..4078)
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(4095..4691)
/label="PuroR"
/note="puromycin N-acetyltransferase"
promoter complement(4700..5029)
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS complement(5043..5786)
/label="Tet-On(R) 3G"
/note="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
promoter complement(5805..6315)
/label="hPGK promoter"
/note="human phosphoglycerate kinase 1 promoter"
promoter 6332..6699
/label="TRE3GS promoter"
/note="3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On(R) 3G, modified to eliminate
binding sites for endogenous mammalian transcription
factors"
CDS 6712..7428
/label="EGFP"
/note="enhanced GFP"
misc_feature complement(7789..7906)
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(7978..8019)
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
CDS complement(8168..8212)
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
misc_feature complement(8397..8630)
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature complement(9127..9252)
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
LTR complement(9299..9932)
/label="3' LTR"
/note="3' long terminal repeat (LTR) from HIV-1"
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