Basic Vector Information
- Vector Name:
- pLV3-H1-2O2-MCS-shRNA-mCherry-TetR-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11890 bp
- Type:
- RNA interference, Tetracycline inducible
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Puro
- Promoter:
- hPGK
- Growth Temperature:
- 37℃
pLV3-H1-2O2-MCS-shRNA-mCherry-TetR-Puro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV3-H1-2O2-MCS-shRNA-mCherry-TetR-Puro vector Sequence
LOCUS 62056_15570 11890 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11890)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..11890
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 7..507
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
intron 516..1088
/label=beta-globin intron
/note="intron from rabbit beta-globin gene"
promoter 1143..1161
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1172..1792
/label=TetR
/note="tetracycline repressor TetR"
misc_feature 1827..2400
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 2420..3016
/label=PuroR
/note="puromycin N-acetyltransferase"
LTR 3147..3380
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 3452..3586
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 3613..3748
/label=SV40 ori
/note="SV40 origin of replication"
promoter complement(3769..3787)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin 3955..4410
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4436..4540
/label=AmpR promoter
CDS 4541..5398
/label=AmpR
/note="beta-lactamase"
rep_origin 5572..6160
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 6448..6469
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6484..6514
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter 6583..6601
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 6629..6855
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
misc_feature 7083..7208
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7701..7934
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8119..8163
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 8312..8353
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
promoter 8452..8546
/label=H1-2O2 promoter
/note="doxycycline-inducible variant of the human H1 RNA
promoter (Henriksen et al., 2007)"
promoter 10000..10507
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
promoter 10735..10938
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 10972..11679
/label=mCherry
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
misc_feature 11721..11838
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
This page is informational only.