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pMSP3535-CRE vector (V014930)

Basic Vector Information

Vector Name:
pMSP3535-CRE
Length:
9349 bp
Replication origin:
ori

pMSP3535-CRE vector Vector Map

pMSP3535-CRE9349 bp400800120016002000240028003200360040004400480052005600600064006800720076008000840088009200ermBPnisKoriT7 promoterCre

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMSP3535-CRE vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_17365        9349 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             332..1066
                     /gene="ermBP"
                     /label=ermBP
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             5245..6585
                     /gene="nisK"
                     /label=nisK
                     /note="Nisin biosynthesis sensor protein NisK from
                     Lactococcus lactis subsp. lactis. Accession#: P42707"
     rep_origin      7090..7678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        7936..7954
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(7973..9001)
                     /label=Cre
                     /note="site-specific recombinase"

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