Basic Vector Information
- Vector Name:
- pLV2-TRE3GV-MCS-mCherry-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8586 bp
- Replication origin:
- ori
- Promoter:
- mPGK
pLV2-TRE3GV-MCS-mCherry-Puro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV2-TRE3GV-MCS-mCherry-Puro vector Sequence
LOCUS V014898 8586 bp DNA circular SYN 01-JAN-1980 DEFINITION Exported. ACCESSION V014898 VERSION V014898 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 8586) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..8586 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 1..634 /label="3' LTR" /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /label="gp41 peptide" /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 1914..1955 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" /label="Protein Tat" misc_feature 2027..2144 /label="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" promoter 2206..2555 /label="TRE3GV promoter" /note="3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On(R) 3G, optimized for retroviral and lentiviral vectors" promoter 2604..3103 /label="PGK promoter" /note="mouse phosphoglycerate kinase 1 promoter" CDS 3131..3838 /label="mCherry" /note="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" CDS 3902..4498 /label="PuroR" /note="puromycin N-acetyltransferase" misc_feature 4508..5096 /label="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 5304..5937 /label="5' LTR" /note="5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6066..6082) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind complement(6090..6106) /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6114..6144) /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind complement(6159..6180) /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(6468..7053) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7227..8084) /label="AmpR" /note="beta-lactamase" promoter complement(8085..8189) /label="AmpR promoter" polyA_signal 8237..8371 /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal"
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