Basic Vector Information
- Vector Name:
- pLV2-TRE3GS-MCS-TetOne-CopGFP-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10066 bp
- Replication origin:
- ori
- Promoter:
- hPGK
pLV2-TRE3GS-MCS-TetOne-CopGFP-Puro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV2-TRE3GS-MCS-TetOne-CopGFP-Puro vector Sequence
LOCUS V014892 10066 bp DNA circular SYN 01-JAN-1980 DEFINITION Exported. ACCESSION V014892 VERSION V014892 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 10066) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..10066 /mol_type="other DNA" /organism="synthetic DNA construct" LTR 1..634 /label="3' LTR" /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /label="gp41 peptide" /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 1914..1955 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" /label="Protein Tat" misc_feature 2027..2144 /label="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" promoter complement(2528..2895) /label="TRE3GS promoter" /note="3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On(R) 3G, modified to eliminate binding sites for endogenous mammalian transcription factors" promoter 2912..3422 /label="hPGK promoter" /note="human phosphoglycerate kinase 1 promoter" CDS 3441..4184 /label="Tet-On(R) 3G" /note="modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline" promoter 4198..4527 /label="SV40 promoter" /note="SV40 enhancer and early promoter" CDS 4578..5243 /label="CopGFP" /note="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" CDS 5313..5366 /label="T2A" /note="2A peptide from Thosea asigna virus capsid protein" CDS 5376..5972 /label="PuroR" /note="puromycin N-acetyltransferase" misc_feature 5989..6577 /label="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 6784..7417 /label="5' LTR" /note="5' long terminal repeat (LTR) from HIV-1" promoter complement(7594..7624) /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind complement(7639..7660) /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7948..8533) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8707..9564) /label="AmpR" /note="beta-lactamase" promoter complement(9565..9669) /label="AmpR promoter" polyA_signal 9717..9851 /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal"
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