Basic Vector Information
- Vector Name:
- Flag-Gsdmd
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7699 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- SV40
- 5' Primer:
- CMV-F
- Growth Temperature:
- 37℃
Flag-Gsdmd vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Flag-Gsdmd vector Sequence
LOCUS 62056_791 7699 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7699)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7699
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 141..157
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
enhancer 318..697
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 698..901
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 945..968
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
CDS 981..2441
/gene="Gsdmd"
/label=Gsdmd
/note="Gasdermin-D from Mus musculus. Accession#: Q9D8T2"
polyA_signal 2458..3080
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
promoter 3122..3419
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3473..4264
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 4919..5053
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter complement(5059..5077)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5091..5107)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5115..5131)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5139..5169)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5184..5205)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5493..6081)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6255..7112)
/label=AmpR
/note="beta-lactamase"
promoter complement(7113..7217)
/label=AmpR promoter
rep_origin 7244..7699
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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