pNL4-3-HA-R-E- vector (V014776)

MSDS

Basic Vector Information

Vector Name:: pNL4-3-HA-R-E-

Antibiotic Resistance:: Ampicillin

Length:: 14609 bp

Type:: Protein expression

Replication origin:: ori

Host:: Mammalian cells, Lentivirus

Growth Temperature:: 37℃

pNL4-3-HA-R-E- vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNL4-3-HA-R-E- vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_18085       14609 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14609)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1290..1547
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1594..1719
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     CDS             1703..3202
                     /label=HIV-1 gag
                     /note="gag protein from human immunodeficiency virus 1"
     CDS             2998..6006
                     /label=HIV-1 pol
                     /note="pol protein from human immunodeficiency virus 1"
     CDS             5954..6529
                     /gene="vif"
                     /label=vif
                     /note="Virion infectivity factor from Human
                     immunodeficiency virus type 1 group M subtype B (isolate 
                     NY5). Accession#: P12504"
     CDS             6743..6916
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH5). Accession#: P04612"
     CDS             6974..7216
                     /gene="vpu"
                     /label=vpu
                     /note="Protein Vpu from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH10). Accession#: P69699"
     misc_feature    8672..8905
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9090..9134
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             9428..9454
                     /label=NES
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             9554..9580
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     LTR             10149..10406
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(12217..12805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12979..13836)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(13837..13941)
                     /label=AmpR promoter

This page is informational only.