Basic Vector Information
- Vector Name:
- pLV-UBC-HBx(HBV)-EGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10402 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Zeo
- Promoter:
- UbC
- Growth Temperature:
- 37℃
pLV-UBC-HBx(HBV)-EGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV-UBC-HBx(HBV)-EGFP vector Sequence
LOCUS V014723 10402 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V014723
VERSION V014723
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 10402)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..10402
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal 417..550
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter complement(635..665)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(680..701)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(989..1577)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1751..2608)
/label="AmpR"
/note="beta-lactamase"
promoter complement(2609..2713)
/label="AmpR promoter"
enhancer 2979..3358
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 3359..3561
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 3576..3756
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 3803..3928
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 4421..4654
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 4839..4883
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 5032..5073
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 5181..5298
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 5363..6574
/label="UbC promoter"
/note="human ubiquitin C promoter"
CDS 7098..7814
/label="EGFP"
/note="enhanced GFP"
misc_feature 7844..8432
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 9160..9384
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 9430..9858
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 10005..10201
/label="SV40 promoter"
/note="SV40 early promoter"
promoter 10249..10296
/label="EM7 promoter"
/note="synthetic bacterial promoter"
CDS join(10315..10402,1..284)
/label="BleoR"
/note="antibiotic-binding protein"
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