Basic Vector Information
- Vector Name:
- pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12668 bp
- Type:
- Protein expression, Tetracycline inducible
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Blast
- Promoter:
- TRE3GV
- Growth Temperature:
- 37℃
pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3 vector Sequence
LOCUS 62056_13890 12668 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12668)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..12668
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(140..166)
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS complement(167..187)
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
promoter complement(246..595)
/label=TRE3GV promoter
/note="3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On(R) 3G, optimized for
retroviral and lentiviral vectors"
misc_feature complement(738..854)
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(931..972)
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
CDS complement(1121..1165)
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
misc_feature complement(1350..1583)
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature complement(2076..2201)
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
LTR complement(2248..2428)
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(2429..2655)
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
promoter complement(2683..2701)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter complement(2770..2800)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(2815..2836)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3124..3712)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3886..4743)
/label=AmpR
/note="beta-lactamase"
promoter complement(4744..4848)
/label=AmpR promoter
rep_origin complement(4874..5329)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5497..5515
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(5536..5671)
/direction=LEFT
/label=SV40 ori
/note="SV40 origin of replication"
polyA_signal complement(5698..5832)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
LTR complement(5904..6137)
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
CDS complement(6227..6931)
/label=rtTA3
/note="reverse tetracycline transactivator 3"
promoter complement(7063..7265)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer complement(7266..7569)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
polyA_signal complement(7903..7958)
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
CDS complement(8119..8511)
/label=BSD
/note="blasticidin S deaminase"
CDS complement(8512..8565)
/label=T2A
/note="2A peptide from Thosea asigna virus capsid protein"
CDS complement(9487..9807)
/label=DmrB
/note="F36V mutant of FK506-binding protein FKBP12"
misc_feature complement(9817..10403)
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS complement(10528..11235)
/label=mTagBFP2
/note="enhanced monomeric blue fluorescent protein (Subach
et al., 2011)"
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