Basic Vector Information
- Vector Name:
- pLV3-4×NFAT-IL-2-Luc2-EGFP-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9797 bp
- Type:
- Luciferase reporter
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Puro
- Promoter:
- RSV
- Growth Temperature:
- 37℃
pLV3-4×NFAT-IL-2-Luc2-EGFP-Puro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV3-4×NFAT-IL-2-Luc2-EGFP-Puro vector Sequence
LOCUS V014695 9797 bp DNA circular SYN 01-JAN-1980 DEFINITION Exported. ACCESSION V014695 VERSION V014695 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 9797) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..9797 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 6..233 /label="RSV promoter" /note="Rous sarcoma virus enhancer/promoter" misc_feature 458..583 /label="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1076..1309 /label="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1493..1537 /label="gp41 peptide" /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 1686..1727 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" /label="Protein Tat" misc_feature 1804..1921 /label="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" protein_bind complement(2056..2085) /label="NFAT binding site" /note="NFAT binding site from the human interleukin 2 (IL-2) gene (Mattila et al., 1990)" promoter 2093..2206 /label="IL-2 promoter" /note="minimal promoter from the human interleukin-2 gene" CDS 2254..3903 /label="luciferase" /note="firefly luciferase" CDS 3979..4695 /label="EGFP" /note="enhanced GFP" promoter 4728..5226 /label="PGK promoter" /note="mouse phosphoglycerate kinase 1 promoter" CDS 5286..5882 /label="PuroR" /note="puromycin N-acetyltransferase" misc_feature 5892..6480 /label="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 6554..6787 /label="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6859..6993 /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin 6999..7134 /label="SV40 ori" /note="SV40 origin of replication" primer_bind complement(7172..7188) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind complement(7196..7212) /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7220..7250) /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind complement(7265..7286) /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7574..8162) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8336..9193) /label="AmpR" /note="beta-lactamase" promoter complement(9194..9298) /label="AmpR promoter" primer_bind 9772..9788 /label="M13 fwd" /note="common sequencing primer, one of multiple similar variants"
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