Basic Vector Information
- Vector Name:
- 2XMyc-LRRK2-D1994A
- Antibiotic Resistance:
- Kanamycin
- Length:
- 11985 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- CMV
- Growth Temperature:
- 37℃
2XMyc-LRRK2-D1994A vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
2XMyc-LRRK2-D1994A vector Sequence
LOCUS 62056_91 11985 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11985)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..11985
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 81..384
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 385..588
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 634..652
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
protein_bind 786..810
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
CDS 825..8405
/gene="LRRK2"
/label=LRRK2
/note="Leucine-rich repeat serine/threonine-protein kinase
2 from Homo sapiens. Accession#: Q5S007"
protein_bind complement(8427..8451)
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
promoter complement(8501..8519)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 8793..8914
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(8921..9376)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 9403..9505
/label=AmpR promoter
promoter 9509..9866
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 9901..10692
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 10927..10974
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 11303..11891
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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