Basic Vector Information
- Vector Name:
- pLV3-CMV-MCS-mCherry-Puro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8112 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Puro
- Promoter:
- hPGK
- Growth Temperature:
- 37℃
pLV3-CMV-MCS-mCherry-Puro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV3-CMV-MCS-mCherry-Puro vector Sequence
LOCUS 62056_15340 8112 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8112) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..8112 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 131..334 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 357..437 /label=MCS /note="multiple cloning site" CDS 445..1152 /label=mCherry /note="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" misc_feature 1195..1312 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 1361..1871 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" CDS 1893..2489 /label=PuroR /note="puromycin N-acetyltransferase" LTR 2620..2853 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 2925..3059 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 3086..3221 /label=SV40 ori /note="SV40 origin of replication" promoter complement(3242..3260) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3270..3286) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 3428..3883 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3909..4013 /label=AmpR promoter CDS 4014..4871 /label=AmpR /note="beta-lactamase" rep_origin 5045..5633 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 5921..5942 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 5957..5987 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5995..6011 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6019..6035 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 6056..6074 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 6102..6328 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" misc_feature 6556..6681 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 7174..7407 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 7592..7636 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 7785..7826 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509"
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