Basic Vector Information
- Vector Name:
- 2X PRE TK luc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6709 bp
- Type:
- Luciferase reporter
- Replication origin:
- ori
- Host:
- Mammalian cells
- Promoter:
- Mini-TK
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
2X PRE TK luc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
2X PRE TK luc vector Sequence
LOCUS 62056_86 6709 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6709)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6709
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(184..772)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(946..1803)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1804..1908)
/label=AmpR promoter
rep_origin complement(2655..3168)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 3609..3625
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 3794..3856
/label=Mini-TK promoter
/note="minimal herpes simplex virus (HSV) thymidine kinase
promoter"
CDS 3916..5565
/codon_start=1
/label=luciferase
/note="firefly luciferase"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
intron 5699..5764
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 5894..5914
/codon_start=1
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/translation="PKKKRKV"
polyA_signal 6339..6473
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6491..6507)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6515..6531)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6539..6569)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6584..6605)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
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