Basic Vector Information
- Vector Name:
- vYM112_PB-pEF1a-optiPIN2-HA(Hygro)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9590 bp
- Type:
- Protein expression, Transposition
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Hyg
- Promoter:
- EF-1α
- Growth Temperature:
- 30℃
vYM112_PB-pEF1a-optiPIN2-HA(Hygro) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
vYM112_PB-pEF1a-optiPIN2-HA(Hygro) vector Sequence
LOCUS 62056_23490 9590 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9590)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..9590
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(230..818)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(992..1849)
/label=AmpR
/note="beta-lactamase"
promoter complement(1850..1954)
/label=AmpR promoter
rep_origin complement(1980..2435)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 2577..2593
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 3228..4406
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
CDS 4465..6405
/gene="PIN2"
/label=PIN2
/note="Auxin efflux carrier component 2 from Arabidopsis
thaliana. Accession#: Q9LU77"
CDS 6418..6444
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
polyA_signal 6478..6685
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
promoter 6723..7052
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 7101..8123
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
polyA_signal 8249..8370
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9418..9434)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(9442..9458)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9466..9496)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(9511..9532)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
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