Basic Vector Information
- Vector Name:
- Lenti-Hb9-GFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12313 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Promoter:
- SV40
- Growth Temperature:
- 37℃
Lenti-Hb9-GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Lenti-Hb9-GFP vector Sequence
LOCUS V014416 12313 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V014416
VERSION V014416
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 12313)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..12313
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..817
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 1059..1184
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1677..1910
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2095..2139
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 2288..2329
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 2391..2507
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 6138..6176
/label="minimal CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 6208..6924
/label="EGFP"
/note="enhanced GFP"
misc_feature 7120..7708
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 8115..8295
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 8327..8551
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 8597..9025
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 9039..9368
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
promoter 9416..9463
/label="EM7 promoter"
/note="synthetic bacterial promoter"
CDS 9482..9853
/label="BleoR"
/note="antibiotic-binding protein"
promoter complement(10204..10234)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(10249..10270)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(10558..11146)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(11320..12177)
/label="AmpR"
/note="beta-lactamase"
promoter complement(12178..12282)
/label="AmpR promoter"
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