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pMIND-RC1973 vector (V014314)

Basic Vector Information

Vector Name:
pMIND-RC1973
Antibiotic Resistance:
Kanamycin
Length:
7225 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Selection Marker:
Hyg
Promoter:
TetRO
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pMIND-RC1973 vector Vector Map

pMIND-RC19737225 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300660069007200M13 revhygM13 fwdNeoR/KanRoriCAP binding sitelac promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMIND-RC1973 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_16810        7225 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..35
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1355..2350
                     /gene="hyg"
                     /label=hyg
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces 
                     hygroscopicus. Accession#: P09979"
     primer_bind     complement(2363..2379)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5192..5983
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6270..6858
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7146..7167
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7182..7212
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"

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