Basic Vector Information
- Vector Name:
- pMS2-GST
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5957 bp
- Type:
- Gene template
- Replication origin:
- ori
- Host:
- E. coli
- Promoter:
- T3
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pMS2-GST vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMS2-GST vector Sequence
LOCUS 62056_17265 5957 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5957)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5957
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 750..776
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS 777..788
/label=Factor Xa site
/note="Factor Xa recognition and cleavage site"
CDS 1179..1832
/label=GST
/note="glutathione S-transferase from Schistosoma
japonicum"
CDS 1833..1853
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
intron 1912..2484
/label=beta-globin intron
/note="intron from rabbit beta-globin gene"
CDS 2488..2610
/gene="HBB"
/label=HBB
/note="Hemoglobin subunit beta from Colobus guereza.
Accession#: Q9TT33"
polyA_signal 2681..2736
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
promoter complement(3091..3109)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3116..3132)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3274..3729
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3755..3859
/label=AmpR promoter
misc_feature 4044..4423
/label=ISS
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
rep_origin 4892..5480
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5768..5789
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5804..5834
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5842..5858
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5866..5882
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 5903..5921
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
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