Basic Vector Information
- Vector Name:
- pLV3-CMV-SP-HIA-TAT-VP3-V5-Blast
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7364 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Selection Marker:
- Blast
- Promoter:
- SV40
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pLV3-CMV-SP-HIA-TAT-VP3-V5-Blast vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV3-CMV-SP-HIA-TAT-VP3-V5-Blast vector Sequence
LOCUS 62056_15455 7364 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7364)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7364
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 55..76
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 91..121
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 129..145
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 153..169
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 190..208
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 236..462
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 463..643
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 690..815
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1308..1541
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1726..1770
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1919..1960
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
enhancer 2049..2352
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2353..2556
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2741..3103
/gene="VP3"
/label=VP3
/note="Apoptin from Chicken anemia virus (isolate Germany
Cuxhaven-1). Accession#: Q99152"
CDS 3104..3145
/label=V5 tag
/note="epitope tag from simian virus 5"
promoter 3190..3519
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 3567..3614
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 3633..4028
/label=BSD
/note="blasticidin S deaminase"
LTR 4118..4351
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 4423..4557
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 4584..4719
/label=SV40 ori
/note="SV40 origin of replication"
promoter complement(4740..4758)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4768..4784)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4926..5381
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5407..5511
/label=AmpR promoter
CDS 5512..6369
/label=AmpR
/note="beta-lactamase"
rep_origin 6543..7131
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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