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pLac-EGFP-Tac-SUL1-Tet-tetG vector (V014191)

Basic Vector Information

Vector Name:
pLac-EGFP-Tac-SUL1-Tet-tetG
Antibiotic Resistance:
Kanamycin
Length:
6201 bp
Type:
Protein expression
Replication origin:
ori
Host:
E. coli
Promoter:
tet
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pLac-EGFP-Tac-SUL1-Tet-tetG vector Vector Map

pLac-EGFP-Tac-SUL1-Tet-tetG6201 bp30060090012001500180021002400270030003300360039004200450048005100540057006000CAP binding sitelac promoterEGFPtac promotersulItet promoterSV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pLac-EGFP-Tac-SUL1-Tet-tetG vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_13670        6201 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..54
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        69..99
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             138..854
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        871..899
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             929..1765
                     /gene="sulI"
                     /label=sulI
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     promoter        1785..1813
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     polyA_signal    2989..3110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3117..3572)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3599..3703
                     /label=AmpR promoter
     promoter        3705..4062
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4097..4888
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5123..5170
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5499..6087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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