pKT pKT-NB-H2BsfGFP vector (V005181)

LOCUS       40924_27044       10171 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pKT pKT-NB-H2BsfGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Kakimoto T, Tsujimura K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2018) Department of Biological Sciences, Graduate 
            School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, 
            Osaka 560-0043, Japan
REFERENCE   2  (bases 1 to 10171)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (24-SEP-2018) Department of Biological Sciences, Graduate School of 
            Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 
            560-0043, Japan"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(279..303)
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     CDS             complement(826..1614)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      complement(2219..2413)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2482..3552)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(3984..4610)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    5945..6085
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6270..6858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7503..7527)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        7663..7842
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7893..8441
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     terminator      8475..8675
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     primer_bind     complement(8688..8704)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8724..8761
                     /label=multi cloning site
                     /note="multi cloning site"
     misc_feature    9197..9208
                     /label=4x glycine linker
                     /note="4x glycine linker"
     CDS             9209..9922
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     misc_feature    9926..9940
                     /label=multi cloning site
                     /note="multi cloning site"
     regulatory      9941..10134
                     /label=terminator of CaMV 35S
                     /note="terminator of CaMV 35S"
                     /regulatory_class="terminator"