pKS145 vector (V005189)

LOCUS       40924_26994        7570 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pKS145 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Terada Y, Park H-S., Kuroki Y, Fujiyama A.
  TITLE     Cloning vector pKS145
  JOURNAL   Published Only in Database (1998)
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Terada Y, Park H-S., Kuroki Y, Fujiyama A.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-1998) Contact:Asao Fujiyama National Institute of 
            Genetics, Human Genetics; Yata 1111, Mishima 411-8540, Japan
REFERENCE   3  (bases 1 to 7570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (1998)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-1998) Contact:Asao Fujiyama National Institute of Genetics, 
            Human Genetics; Yata 1111, Mishima 411-8540, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Dec 22, 2008 this sequence version replaced AB013921.1.
FEATURES             Location/Qualifiers
     source          1..7570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(73..89)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(87..106)
                     /label=pKS145_Fwd
                     /note="pKS145_Fwd"
     regulatory      complement(881..922)
                     /note="contains the T4 gene 32 terminator and a portion of
                     the polylinker from the omega interposon; omega terminator"
                     /regulatory_class="terminator"
     CDS             940..1797
                     /label=AmpR
                     /note="beta-lactamase"
     misc_feature    complement(2640..3113)
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(3189..4157)
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             complement(4160..5332)
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    complement(5658..5908)
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(5914..6666)
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     rep_origin      complement(6757..6976)
                     /direction=LEFT
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     protein_bind    7396..7417
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7432..7462
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7470..7486
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7494..7510
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7532..7550
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"