pKPFWG2 vector (V005196)

LOCUS       40924_26964       11539 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pKPFWG2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Wang C, Sze H.
  TITLE     Generating binary vectors for pollen-specific expression of proteins
            tagged to eGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11539)
  AUTHORS   Wang C, Sze H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2007) Cell Biology and Molecular Genetics, 
            University of Maryland, H J Patterson Hall Rm 3232, College Park, MD
            20742, USA
REFERENCE   3  (bases 1 to 11539)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11539)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-MAR-2007) Cell Biology and Molecular Genetics, University of 
            Maryland, H J Patterson Hall Rm 3232, College Park, MD 20742, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(7..720)
                     /note="AtCHX08 promoter; promoter region of Arabidopsis
                     thaliana CHX08; drives pollen-specific over-expression in 
                     Arabidopsis thaliana"
                     /regulatory_class="promoter"
     misc_feature    789..813
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2113..2739
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             3176..4240
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      4309..4503
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4847..4987
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5173..5761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6008..6796)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    7324..7348
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(7441..7693)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7736..8527)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(8561..8740)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      complement(8873..9098)
                     /note="T35S; Cauliflower mosaic virus 35S terminator"
                     /regulatory_class="terminator"
     CDS             complement(9105..9821)
                     /label=EGFP
                     /note="enhanced GFP"
     protein_bind    9829..9953
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(9997..10299)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(10644..11300)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(11354..11384)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(11409..11533)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"