pKKM801 vector (V005221)

LOCUS       V005221                 6921 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005221
VERSION     V005221
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Sakka K, Wang YY, Sakka M, Kunitake E, Kimura T.
  TITLE     A plasmid vector for Ruminiclostridium josui
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Sakka K, Kimura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2017) Contact:Kazuo Sakka Mie University, Graduate
            School of Bioresources; 1577 Kurimamachiya-cho, Tsu, Mie 514-8507,
            Japan URL :http://www.bio.mie-u.ac.jp/
REFERENCE   3  (bases 1 to 6921)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6921)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-OCT-2017) Contact:Kazuo Sakka Mie University, Graduate School of
            Bioresources"; volume: " 1577 Kurimamachiya-cho, Tsu, Mie 514-8507,
            Japan URL :http"; pages: "//www.bio.mie-u.ac.jp"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        90..194
                     /label="AmpR promoter"
     CDS             195..1052
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1226..1814
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2102..2123
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2138..2168
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2176..2192
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2200..2216
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      2232..2275
                     /label="pcip promoter"
                     /note="pcip promoter"
                     /regulatory_class="promoter"
     RBS             2558..2566
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2824..2841
                     /label="6xHis"
                     /note="6xHis affinity tag"
     primer_bind     complement(2854..2870)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3458..3687
                     /note="oriCP; C. perfringens pIP404 replication origin"
     CDS             complement(3750..4970)
                     /codon_start=1
                     /gene="rep"
                     /product="pIP404 replication"
                     /label="rep"
                     /protein_id="BBA94093.1"
                     /translation="MRNALKLNKKNYIDNIHSRSKGWITRSVIDKKGYSQWHYKYAELK
                     DLDMSDENIYITLNTFYKPCRRLENIKELNTLFIDLDYYKTGKTKDQVLMDLEKNYFNQ
                     SIPIPNYVIDSGRGMYLIWIINAVPSKALPLWKAVQEYLYNQLKYFGADRQALDATRIL
                     RVPGSINSKSKTVVNILDEYEYIYDLREIQNGFLPELKPYERKKGRPSKINYIYRERSL
                     YYGRIQDIIKLCELREYDLKGHRELILFLYRYYLCSFTEDIEKALNDVLELNSMFRQHL
                     SEREVIRATRSAERCYLDKNKQYKYKNETLIELLEITEEEQKYMTIIISKKEYKRRENI
                     RGKKNYQEQLKAKGKATKKEELNVLRKKIKALKEKGFKNKEITLMLEVPIKTLERHITY
                     MKKNGLL"
     gene            complement(3750..4970)
                     /gene="rep"
                     /label="rep"
     CDS             complement(6032..6766)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"