pKIT303 vector (V005233)

LOCUS       V005233                 4999 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005233
VERSION     V005233
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Toda, H., Itoh, N.
  TITLE     Development of novel E. coli-Kocuria shuttle vector using cryptic
            plasmid pKPAL3 from Kocuria palustris IPUFS-1 and utilization for
            producing enantiopure (S)-styrene oxide
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Itoh N, Toda, H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2017) Contact:Nobuya Itoh Toyama Prefectural
            University, Biotechnology Research Center; 5180 Kurokawa, Imizu,
            Toyama 939-0398, Japan URL :http://www.pu-toyama.ac.jp/BR/itoh/
REFERENCE   3  (bases 1 to 4999)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4999)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-AUG-2017) Contact:Nobuya Itoh Toyama Prefectural University,
            Biotechnology Research Center"; volume: " 5180 Kurokawa, Imizu,
            Toyama 939-0398, Japan URL :http"; pages:
            "//www.pu-toyama.ac.jp/BR/itoh"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    39..60
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        75..105
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    113..129
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     137..153
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    163..219
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(223..239)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      452..907
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1189..1293
                     /label="AmpR promoter"
     CDS             1294..2151
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2325..2913
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3141..3947
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     rep_origin      3996..4999
                     /label="pKPAL3-derived plasmid replication origin"
                     /note="pKPAL3-derived plasmid replication origin"