pKIL-HIS3 vector (V005236)

LOCUS       40924_26719        4806 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pKIL-HIS3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Van Reeth T, Dreze PL, Szpirer J, Szpirer C, Gabant P.
  TITLE     Positive selection vectors to generate fused genes for the 
            expression of his-tagged proteins
  JOURNAL   BioTechniques 25 (5), 898-904 (1998)
  PUBMED    9821593
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Van Reeth T, Gabant P, Dreze PL, Szpirer J, Szpirer C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-1998) Departement de Biologie Moleculaire, 
            Universite Libre de Bruxelles, 67, rue des Chevaux, Rhode St. Genese
            1640, Belgium
REFERENCE   3  (bases 1 to 4806)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4806)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1998"; volume: "25"; issue: "5"; pages: 
            "898-904"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-FEB-1998) Departement de Biologie Moleculaire, Universite Libre 
            de Bruxelles, 67, rue des Chevaux, Rhode St. Genese 1640, Belgium"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..59
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    67..83
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    100..169
                     /label=rrnG antiterminator
                     /note="antiterminator from the E. coli rrnG leader region
                     (Berg et al., 1989)"
     CDS             220..243
                     /label=minicistron
                     /note="synthetic cistron containing a ribosome binding site
                     (Shine-Dalgarno sequence), for enhancing the bacterial 
                     expression of a downstream cistron (Schoner, 1997)"
     CDS             257..271
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     CDS             281..298
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             472..771
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      876..1002
                     /gene="ccdB"
                     /regulatory_class="terminator"
     terminator      996..1082
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1174..1201
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1221..1312
                     /label=AmpR promoter
     CDS             1313..2170
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2344..2932
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3118..3258)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        3444..3521
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3522..4601
                     /label=lacI
                     /note="lac repressor"