pJKPN vector (V005319)

LOCUS       V005319                 7456 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005319
VERSION     V005319
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Gong Z, Lv H.
  TITLE     Vector pJKPN for targeted integration at the Leu1-32 of
            Schizosaccharomyces pombe
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Gong Z, Lv H.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2011) School of Life Science, Fudan University,
            Room 618, Science Building, Fudan University, Handan Road 220,
            Shanghai, Shanghai 200433, China
REFERENCE   3  (bases 1 to 7456)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7456)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-SEP-2011) School of Life Science, Fudan University, Room 618,
            Science Building, Fudan University, Handan Road 220, Shanghai,
            Shanghai 200433, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           187..306
                     /gene="Leu1"
     CDS             307..1419
                     /gene="leu1"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from
                     Schizosaccharomyces pombe (strain 972 / ATCC 24843).
                     Accession#: P18869"
     3'UTR           1423..2258
                     /gene="Leu1"
     rep_origin      complement(2308..2763)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2910..2926
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2936..2954
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2995..4159
                     /label="nmt1 promoter"
                     /note="wild-type nmt1 promoter from Schizosaccharomyces
                     pombe, conferring strong thiamine-repressible expression"
     regulatory      4181..5177
                     /label="NMT1 terminator"
                     /note="NMT1 terminator"
                     /regulatory_class="terminator"
     promoter        complement(5196..5214)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5235..5251)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5259..5275)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5283..5313)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5328..5349)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5637..6225)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6399..7256)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7257..7361)
                     /label="AmpR promoter"