pJH370 vector (V005331)

LOCUS       40924_26106        5141 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJH370, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Hu JC, O'Shea EK, Kim PS, Sauer RT.
  TITLE     Sequence requirements for coiled-coils: analysis with lambda 
            repressor-GCN4 leucine zipper fusions
  JOURNAL   Science 250 (4986), 1400-1403 (1990)
  PUBMED    2147779
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Hu JC.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5141)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5141)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "1990"; volume: "250"; issue: "4986"; pages: "1400-1403"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-OCT-2000) Biochemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..58
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    66..82
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      91..94
                     /regulatory_class="ribosome_binding_site"
     CDS             535..600
                     /label=GCN4_v1
                     /note="GCN4 peptide that allows binding to an scFv-GFP
                     fusion protein (Tanenbaum et al., 2014)"
     terminator      670..717
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             2202..2390
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2495..2635
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2821..3409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3520..4033
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(4078..4935)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4936..5040)
                     /label=AmpR promoter