pIZT-W(Gm) vector (V005387)

LOCUS       40924_25821        4594 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pIZT-W(Gm) DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Tatsuke T, Lee J, Iiyama K, Sezutsu H, Uchino K, Kusakabe T.
  TITLE     Tightly controlled Tetracycline-inducible transcription system for 
            explosive gene expression in cultured silkworm cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Tatsuke T, Kusakabe T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2012) Contact:Takahiro Kusakabe Graduate School of
            Bioresource and Bioenvironmental Sciences, Kyushu University, 
            Laboratory of Silkworm Science; 6-10-1 Hakozaki, Higashi-ku, 
            Fukuoka, Fukuoka 812-8581, Japan
REFERENCE   3  (bases 1 to 4594)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4594)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-APR-2012) Contact:Takahiro Kusakabe Graduate School of 
            Bioresource and Bioenvironmental Sciences, Kyushu University, 
            Laboratory of Silkworm Science; 6-10-1 Hakozaki, Higashi-ku, 
            Fukuoka, Fukuoka 812-8581, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..548
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     protein_bind    647..771
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        796..826
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             880..1536
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1881..2183
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2227..2351)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2386..2427
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             2437..2454
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    2472..2601
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(2729..3317)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3773..3801
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3990..4520
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"