pIRIA vector (V005402)

LOCUS       V005402                11038 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005402
VERSION     V005402
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Wojcik EA, Brzostek A, Bacolla A, Mackiewicz P, Vasquez KM,
            Korycka-Machala M, Jaworski A, Dziadek J.
  TITLE     Direct and inverted repeats elicit genetic instability by both
            exploiting and eluding DNA double-strand break repair systems in
            mycobacteria
  JOURNAL   PLoS ONE 7 (12), E51064 (2012)
   PUBMED   23251422
REFERENCE   2  (bases 1 to 11038)
  AUTHORS   Dziadek J, Wojcik EA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2012) Pharmacology and Toxicology, University of
            Texas, 1400 Barbara Jordan Blvd., Austin, TX 78759, USA
REFERENCE   3  (bases 1 to 11038)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11038)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2012"; volume: "7"; issue: "12"; pages: "E51064"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-OCT-2012) Pharmacology and Toxicology, University of Texas, 1400
            Barbara Jordan Blvd., Austin, TX 78759, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             511..1623
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     CDS             1907..2719
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3054..3642
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     repeat_region   4965..5004
                     /rpt_family="IR-1"
     CDS             complement(5125..5655)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(5844..5872)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     repeat_region   5897..5932
                     /rpt_family="IR-2"
     promoter        6687..6705
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             6995..10039
                     /label="lacZ"
                     /note="beta-galactosidase"
     terminator      10956..11002
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"