pIREA vector (V005404)

LOCUS       40924_25651       10070 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pIREA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Wojcik EA, Brzostek A, Bacolla A, Mackiewicz P, Vasquez KM, 
            Korycka-Machala M, Jaworski A, Dziadek J.
  TITLE     Direct and inverted repeats elicit genetic instability by both 
            exploiting and eluding DNA double-strand break repair systems in 
            mycobacteria
  JOURNAL   PLoS ONE 7 (12), E51064 (2012)
  PUBMED    23251422
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   Dziadek J, Wojcik EA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2012) Pharmacology and Toxicology, University of 
            Texas, 1400 Barbara Jordan Blvd., Austin, TX 78759, USA
REFERENCE   3  (bases 1 to 10070)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10070)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "12"; pages: "E51064"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-OCT-2012) Pharmacology and Toxicology, University of Texas, 1400
            Barbara Jordan Blvd., Austin, TX 78759, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      complement(2028..3927)
                     /direction=LEFT
                     /label=mycobacterial OriM region from pAL5000
                     /note="mycobacterial OriM region from pAL5000"
     CDS             4201..7245
                     /label=lacZ
                     /note="beta-galactosidase"
     repeat_region   8411..8450
                     /rpt_family="IR-1"
     CDS             complement(8571..9101)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(9290..9318)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   9343..9378
                     /rpt_family="IR-2"
     terminator      complement(9991..10034)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"