pIPKb003 vector (V005410)

LOCUS       40924_25621       13863 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pIPKb003, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13863)
  AUTHORS   Himmelbach A, Zierold U, Hensel G, Riechen J, Douchkov D, Schweizer 
            P, Kumlehn J.
  TITLE     A set of modular binary vectors for transformation of cereals
  JOURNAL   Plant Physiol. 145 (4), 1192-1200 (2007)
  PUBMED    17981986
REFERENCE   2  (bases 1 to 13863)
  AUTHORS   Himmelbach A, Zierold U, Hensel G, Riechen J, Douchkov D, Schweizer 
            P, Kumlehn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2007) Cytogenetics and Molecular Cell Biology, 
            Leibniz Institute of Plant Genetics and Crop Plant Research, 
            Corrensstr. 3, Gatersleben D-06466, Germany
REFERENCE   3  (bases 1 to 13863)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13863)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1192-1200"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-SEP-2007) Cytogenetics and Molecular Cell Biology, Leibniz 
            Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 
            Gatersleben D-06466, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      10..996
                     /label=maize ubiquitin (Ubi-1) promoter
                     /note="maize ubiquitin (Ubi-1) promoter"
                     /regulatory_class="promoter"
     intron          997..1516
                     /note="maize ubiquitin (Ubi-1) intron 1"
     CDS             1544..2566
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      2594..2795
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     regulatory      2828..2837
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      complement(2848..3100)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    3119..3243
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3287..3589)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(3934..4590)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4644..4674)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(4699..4823)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5378..5395)
                     /label=6xHis
                     /note="6xHis affinity tag"
     promoter        complement(5398..6230)
                     /label=Act1 promoter
                     /note="rice actin promoter"
     misc_feature    6337..6361
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7006..7594
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7779..7919)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             9254..9880
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             10312..11382
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      11451..11645
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             12250..13038
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    13561..13585
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"