Basic Vector Information
- Vector Name:
- pINDEX4
- Antibiotic Resistance:
- Kanamycin
- Length:
- 12072 bp
- Type:
- Binary vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Ouwerkerk PB, de Kam RJ, Hoge JH, Meijer AH.
- Promoter:
- CaMV 35S (enhanced)
pINDEX4 vector Map
pINDEX4 vector Sequence
LOCUS 40924_25511 12072 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary vector pINDEX4, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12072)
AUTHORS Ouwerkerk PB, de Kam RJ, Hoge JH, Meijer AH.
TITLE Glucocorticoid-inducible gene expression in rice
JOURNAL Planta 213 (3), 370-378 (2001)
PUBMED 11506359
REFERENCE 2 (bases 1 to 12072)
AUTHORS Ouwerkerk PBF.
TITLE Direct Submission
JOURNAL Submitted (11-AUG-2000) Dept. of Molecular Cell Biology, Institute
for Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL,
Netherlands
REFERENCE 3 (bases 1 to 12072)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12072)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Planta";
date: "2001"; volume: "213"; issue: "3"; pages: "370-378"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(11-AUG-2000) Dept. of Molecular Cell Biology, Institute for
Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL,
Netherlands"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12072
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 36..74
/note="multiple cloning site; restriction sites
HpaI-ApaI-MunI-MluI-SwaI are unique. In here a promoter of
choice to drive GVG expression can be inserted."
CDS 321..554
/label=VP16 AD
/note="transcriptional activation domain of herpes simplex
virus protein VP16 (Triezenberg et al., 1988; Cousens et
al., 1989)"
3'UTR 1421..1717
/label=Pisum sativum RbcS-E9 polyA signal
/note="Pisum sativum RbcS-E9 polyA signal"
terminator 1895..2147
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
regulatory 2199..2269
/note="4UAS; tetramer of the GVG binding site"
/regulatory_class="promoter"
promoter 2318..2364
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
misc_feature 2395..2452
/note="multiple cloning site; restriction sites
BbrPI-SpeI-Sse8387I-XhoI-Bsp1407I-StuI are unique."
3'UTR 2456..2924
/label=Pisum sativum RbcS-3A polyA signal
/note="Pisum sativum RbcS-3A polyA signal"
primer_bind complement(2963..2979)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 3182..3206
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 4506..5132
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 5569..6633
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 6702..6896
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 7240..7380
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(7566..8154)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8244..9035)
/label=KanR
/note="aminoglycoside phosphotransferase"
misc_feature 9460..9484
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal complement(9562..9736)
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
exon complement(9787..10439)
/gene="hptII"
/number=2
intron complement(10474..10663)
/label=cat1 intron
/note="castor bean catalase intron, modified"
exon complement(10670..11002)
/gene="hptII"
/number=1
promoter complement(11081..11758)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 11949..11970
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 11985..12015
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 12023..12039
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 12047..12063
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
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