pINDEX2 vector (V005430)

LOCUS       40924_25501       14230 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pINDEX2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14230)
  AUTHORS   Ouwerkerk PB, de Kam RJ, Hoge JH, Meijer AH.
  TITLE     Glucocorticoid-inducible gene expression in rice
  JOURNAL   Planta 213 (3), 370-378 (2001)
  PUBMED    11506359
REFERENCE   2  (bases 1 to 14230)
  AUTHORS   Ouwerkerk PBF.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2000) Dept. of Molecular Cell Biology, Institute 
            for Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands
REFERENCE   3  (bases 1 to 14230)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14230)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Planta"; 
            date: "2001"; volume: "213"; issue: "3"; pages: "370-378"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-AUG-2000) Dept. of Molecular Cell Biology, Institute for 
            Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      28..2233
                     /note="constitutive; derived from the Oryza sativa Gos2
                     encoded by Genbank Accession Number X51910"
                     /regulatory_class="promoter"
     exon            1058..1194
                     /number=1
     intron          1195..2157
                     /number=1
                     /note="derived from Gos2"
     CDS             2479..2712
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
     3'UTR           3579..3875
                     /label=Pisum sativum RbcS-E9 polyA signal
                     /note="Pisum sativum RbcS-E9 polyA signal"
     terminator      4053..4305
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      4357..4427
                     /note="4UAS; tetramer of the GVG binding site"
                     /regulatory_class="promoter"
     promoter        4476..4522
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    4553..4610
                     /note="multiple cloning site; 
                     BbrPI-SpeI-Sse8387I-XhoI-Bsp1407I-StuI are unique."
     3'UTR           4614..5082
                     /label=Pisum sativum RbcS-3A polyA signal
                     /note="Pisum sativum RbcS-3A polyA signal"
     primer_bind     complement(5121..5137)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5340..5364
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6664..7290
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             7727..8791
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      8860..9054
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9398..9538
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9724..10312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10402..11193)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    11618..11642
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(11720..11894)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     exon            complement(11945..12637)
                     /gene="hptII"
                     /number=2
     intron          complement(12632..12821)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     exon            complement(12828..13160)
                     /gene="hptII"
                     /number=1
     promoter        complement(13239..13916)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    14107..14128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        14143..14173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14181..14197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14205..14221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"