pILL2150 vector (V005435)

LOCUS       40924_25461       11198 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Inducible expression vector pILL2150, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Boneca IG, Ecobichon C, Chaput C, Mathieu A, Guadagnini S, Prevost 
            MC, Colland F, Labigne A, de Reuse H.
  TITLE     Development of inducible systems to engineer conditional mutants of 
            essential genes of Helicobacter pylori
  JOURNAL   Appl. Environ. Microbiol. 74 (7), 2095-2102 (2008)
  PUBMED    18245237
REFERENCE   2  (bases 1 to 11198)
  AUTHORS   Boneca IG, Ecobichon C, de Reuse H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2008) Microbiology, Institut Pasteur, 28 rue du 
            Dr. Roux, Paris 75724, France
REFERENCE   3  (bases 1 to 11198)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11198)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "7"; pages:
            "2095-2102"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2008) Microbiology, Institut Pasteur, 28 rue du Dr. Roux, 
            Paris 75724, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(168..189)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(205..1284)
                     /label=lacI
                     /note="lac repressor"
     regulatory      complement(1285..1536)
                     /label=amiE promoter region
                     /note="amiE promoter region"
                     /regulatory_class="promoter"
     CDS             4546..5202
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      5334..5382
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     misc_feature    5396..5421
                     /label=multicloning site
                     /note="multicloning site"
     CDS             complement(5425..8493)
                     /label=lacZ
                     /note="beta-galactosidase"
     protein_bind    8572..8588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8596..8624)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    complement(8833..8854)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(8870..9949)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(9950..10027)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     rep_origin      complement(10219..10807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            11033..11142
                     /label=oriT
                     /note="incP origin of transfer"