Basic Vector Information
phRG(R2.2) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
phRG(R2.2) vector Sequence
LOCUS 40924_24947 4304 bp DNA circular SYN 18-DEC-2018 DEFINITION Reporter vector phRG(R2.2), complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4304) AUTHORS Almond BD, Chauvin F, Kenefick KB. TITLE Rapid Response (TM) Reporter Vectors Technical Manual, TM242 JOURNAL Unpublished REFERENCE 2 (bases 1 to 4304) AUTHORS Almond BD, Chauvin F, Kenefick KB. TITLE Direct Submission JOURNAL Submitted (26-NOV-2003) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA REFERENCE 3 (bases 1 to 4304) TITLE Direct Submission REFERENCE 4 (bases 1 to 4304) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (26-NOV-2003) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4304 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..58 /label=multiple cloning region /note="multiple cloning region" CDS 88..1020 /codon_start=1 /label=hRluc /note="Renilla luciferase" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" CDS 1027..1074 /codon_start=1 /label=hCL1 /note="non-ORF yeast peptide conferring ubiquitin-dependent degradation" /translation="ACKNWFSSLSHFVIHL" CDS 1078..1197 /codon_start=1 /label=hPEST /note="PEST degradation sequence from mouse ornithine decarboxylase" /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV" polyA_signal complement(1267..1388) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind 1547..1566 /label=reporter vector primer 4 (RVPrimer4) /note="reporter vector primer 4 (RVPrimer4)" rep_origin 1804 /label=ColE1-derived /note="ColE1-derived" rep_origin complement(1807..2395) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2569..3426) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3427..3531) /label=AmpR promoter rep_origin 3558..4013 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" polyA_signal 4144..4192 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 4206..4297 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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