pHR50 vector (V005505)

LOCUS       V005505                11973 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005505
VERSION     V005505
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11973)
  AUTHORS   Ramos HJO., Soares-Ramos JRL., Souza EM, Pedrosa FO.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2003) Department of Biochemistry and Molecular
            Biology, Universidade Federal do Parana - UFPR, Centro Politecnico,
            Curitiba, Parana 81531-990, Brazil
REFERENCE   2  (bases 1 to 11973)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11973)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (14-FEB-2003) Department of Biochemistry and Molecular Biology,
            Universidade Federal do Parana - UFPR, Centro Politecnico, Curitiba,
            Parana 81531-990, Brazil"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             1793..2452
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     primer_bind     2614..2630
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(2985..3776)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(4709..6517)
                     /label="GUS"
                     /note="beta-glucuronidase"
     primer_bind     complement(6555..6571)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(6613..7326)
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     CDS             complement(7379..8950)
                     /gene="nifA"
                     /label="Nif-specific regulatory protein"
                     /note="Nif-specific regulatory protein from Klebsiella
                     pneumoniae. Accession#: P03027"
     regulatory      complement(9520..9525)
                     /note="derived from gentamycin resistance aacC1 gene of
                     pJQ200KS"
                     /regulatory_class="minus_10_signal"
     promoter        complement(9525..9553)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     protein_bind    10062..10083
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10098..10128
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10136..10152
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10160..10176
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        10197..10215
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     10245..10261
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(10905..11762)
                     /label="AmpR"
                     /note="beta-lactamase"