pHR'-CMVLacZ vector (V005514)

LOCUS       V005514                11686 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005514
VERSION     V005514
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Naldini L, Blomer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM,
            Trono D.
  TITLE     In vivo gene delivery and stable transduction of nondividing cells
            by a lentiviral vector
  JOURNAL   Science 272 (5259), 263-267 (1996)
   PUBMED   8602510
REFERENCE   2  (bases 1 to 11686)
  AUTHORS   Zufferey R, Dull T, Mandel RJ, Bukovsky A, Quiroz D, Naldini L,
            Trono D.
  TITLE     Self-inactivating lentivirus vector for safe and efficient in vivo
            gene delivery
  JOURNAL   J. Virol. 72 (12), 9873-9880 (1998)
   PUBMED   9811723
REFERENCE   3  (bases 1 to 11686)
  AUTHORS   Cherepanov P, Claeys A, Trono D, Debyser Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-1998) AIDS Laboratory, Rega Institute for Medical
            Research, Minderbroedersstr. 10, Leuven 3000, Belgium
REFERENCE   4  (bases 1 to 11686)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11686)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science";
            date: "1996"; volume: "272"; issue: "5259"; pages: "263-267"
            SGRef: number: 2; type: "Journal Article"; journalName: "J. Virol.";
            date: "1998"; volume: "72"; issue: "12"; pages: "9873-9880"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (09-NOV-1998) AIDS Laboratory, Rega Institute for Medical Research,
            Minderbroedersstr. 10, Leuven 3000, Belgium"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..636
     LTR             3..636
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(661..679)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1467..1571
                     /label="AmpR promoter"
     CDS             1572..2429
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2603..3191
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      3197
                     /label="pUC replication origin"
                     /note="pUC replication origin"
     promoter        3437..3766
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    3966..4423
                     /gene="gpt"
                     /note="gpt open reading frame disrupted by a frame shift
                     resulting in loss of the KpnI site in the gpt gene"
     gene            3966..4423
                     /gene="gpt"
                     /label="gpt"
                     /note="xanthine guanine phosphoribosyl transferase"
     intron          4900..4965
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5095..5115
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    5540..5674
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             5843..6476
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6523..6648
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6674..6675
                     /note="gag ORF disrupted by a frame shift; ClaI site
                     filled-in"
     misc_feature    7141..7374
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             7559..7603
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     misc_feature    7748..7750
                     /label="3'-splice site"
                     /note="3'-splice site"
     CDS             7752..7793
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        7882..8185
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8186..8389
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             8518..11562
                     /label="lacZ"
                     /note="beta-galactosidase"