pHCMV-SARS-S (FFM-1) vector (V005577)

LOCUS       V005577                 8628 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005577
VERSION     V005577
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Giroglou T, Cinatl J Jr., Rabenau H, Drosten C, Schwalbe H, Doerr
            HW, von Laer D.
  TITLE     Retroviral vectors pseudotyped with severe acute respiratory
            syndrome coronavirus S protein
  JOURNAL   J. Virol. 78 (17), 9007-9015 (2004)
   PUBMED   15308697
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   Giroglou T.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2004) Applied Virology and Genetherapy,
            Georg-Speyer-Haus, Paul-Ehrlich-Str. 42-44, Frankfurt 60596, Germany
REFERENCE   3  (bases 1 to 8628)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8628)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2004"; volume: "78"; issue: "17"; pages: "9007-9015"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAR-2004) Applied Virology and Genetherapy, Georg-Speyer-Haus,
            Paul-Ehrlich-Str. 42-44, Frankfurt 60596, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..462
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          778..1350
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             1420..5184
                     /gene="S"
                     /label="Spike glycoprotein"
                     /note="Spike glycoprotein from Severe acute respiratory
                     syndrome coronavirus. Accession#: P59594"
     polyA_signal    5384..5439
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(5797..5813)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5821..5837)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5845..5875)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5890..5911)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6199..6787)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6961..7818)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7819..7923)
                     /label="AmpR promoter"
     rep_origin      complement(7949..8404)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8546..8562
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8572..8590
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"