pMON74532 vector (V004682)

LOCUS       V004682                 9754 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004682
VERSION     V004682
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil
            VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R,
            Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
  TITLE     Expression of the Arabidopsis thaliana BBX32 Gene in Soybean
            Increases Grain Yield
  JOURNAL   PLoS ONE 7 (2), E30717 (2012)
   PUBMED   22363475
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Preuss SB.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9754)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9754)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2012"; volume: "7"; issue: "2"; pages: "E30717"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JUL-2011) Yield "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    51..75
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    complement(504..1392)
                     /label="derived from E. coli aadA"
                     /note="derived from E. coli aadA"
     CDS             complement(562..1350)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label="SmR"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1923..2511
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2697..2837)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2942..3130)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     rep_origin      4398..5111
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    5277..5301
                     /label="LB T-DNA repeat"
                     /note="left border repeat from octopine Ach5 T-DNA"
     regulatory      5602..6201
                     /label="derived from A. thaliana Act7"
                     /note="derived from A. thaliana Act7"
                     /regulatory_class="promoter"
     5'UTR           6202..6839
                     /label="derived from A. thaliana Act7"
                     /note="derived from A. thaliana Act7"
     CDS             6842..7069
                     /codon_start=1
                     /product="EPSPS CTP"
                     /label="EPSPS CTP"
                     /note="derived from A. thaliana ShkG"
                     /protein_id="AEP17833.1"
                     /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI
                     SSSWGLKKSGMTLIGSELRPLKVMSSVSTAC"
     CDS             7070..8434
                     /gene="aroA"
                     /label="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /note="3-phosphoshikimate 1-carboxyvinyltransferase from
                     Agrobacterium sp. (strain CP4). Accession#: Q9R4E4"
     terminator      8444..8696
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        9018..9362
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     3'UTR           9420..9734
                     /label="derived from G. barbadense E6"
                     /note="derived from G. barbadense E6"