Basic Vector Information
- Vector Name:
- pMON108080
- Antibiotic Resistance:
- Streptomycin
- Length:
- 10566 bp
- Type:
- Expression vector
- Replication origin:
- oriV
- Host:
- Plants
- Source/Author:
- Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R, Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
- Promoter:
- CaMV 35S
pMON108080 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMON108080 vector Sequence
LOCUS 40924_31205 10566 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pMON108080, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10566)
AUTHORS Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil
VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R,
Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
TITLE Expression of the Arabidopsis thaliana BBX32 Gene in Soybean
Increases Grain Yield
JOURNAL PLoS ONE 7 (2), E30717 (2012)
PUBMED 22363475
REFERENCE 2 (bases 1 to 10566)
AUTHORS Preuss SB.
TITLE Direct Submission
JOURNAL
REFERENCE 3 (bases 1 to 10566)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10566)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2012"; volume: "7"; issue: "2"; pages: "E30717"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-JUL-2011) Yield "
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10566
/mol_type="other DNA"
/organism="synthetic DNA construct"
3'UTR 31..345
/label=derived from G. barbadense E6
/note="derived from G. barbadense E6"
misc_feature 416..440
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
misc_feature complement(869..1757)
/label=derived from E. coli aadA
/note="derived from E. coli aadA"
CDS complement(927..1715)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 2288..2876
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(3062..3202)
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3307..3495)
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
rep_origin 4763..5476
/label=oriV
/note="incP origin of replication"
misc_feature 5642..5666
/label=LB T-DNA repeat
/note="left border repeat from octopine Ach5 T-DNA"
regulatory 5967..6566
/label=derived from A. thaliana Act7
/note="derived from A. thaliana Act7"
/regulatory_class="promoter"
5'UTR 6567..7204
/label=derived from A. thaliana Act7
/note="derived from A. thaliana Act7"
CDS 7207..7434
/codon_start=1
/product="EPSPS CTP"
/label=EPSPS CTP
/note="derived from A. thaliana ShkG"
/protein_id="AEP17820.1"
/translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI
SSSWGLKKSGMTLIGSELRPLKVMSSVSTAC"
CDS 7435..8799
/gene="aroA"
/label=aroA
/note="3-phosphoshikimate 1-carboxyvinyltransferase from
Agrobacterium sp. (strain CP4). Accession#: Q9R4E4"
terminator 8809..9061
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter 9348..9693
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
5'UTR 9701..9789
/label=derived from P. hybrida DnaK
/note="derived from P. hybrida DnaK"
CDS 9836..10561
/codon_start=1
/product="B-box 52 protein"
/label=B-box 52 protein
/note="derived from G. max BBX52"
/protein_id="AEP17822.1"
/translation="MKGKTCELCDQQASLYCPSDSAFLCSDCDAAVHAANFLVARHLRR
LLCSKCNRFAGFHISSGAISRHLSSTCSSCSPENPSADYSDSLPSSSTCVSSSESCSTK
QIKVEKKRSWSGSSVTDDASPAAKKRQRSGGSEEVFEKWSREIGLGLGLGVNGNRVASN
ALSVCLGKWRWLPFRVAAATSFWLGLRFCGDRGLASCQNLARLEAISGVPVKLILAAHG
DLARVFTHRRELQEGWGES"
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