pMO126 vector (V004689)

MSDS

Basic Vector Information

Vector Name:: pMO126

Antibiotic Resistance:: Kanamycin

Length:: 4788 bp

Type:: Insertional disruption vector

Replication origin:: ori

Source/Author:: Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI, Vazquez-Torres A.

Promoter:: tac

pMO126 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMO126 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_31170        4788 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Insertional disruption vector pMO126, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Jones-Carson J, Laughlin J, Hamad MA, Stewart AL, Voskuil MI, 
            Vazquez-Torres A.
  TITLE     Inactivation of [Fe-S] metalloproteins mediates nitric 
            oxide-dependent killing of Burkholderia mallei
  JOURNAL   PLoS ONE 3 (4), E1976 (2008)
  PUBMED    18398486
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Jones-Carson J, Laughlin J, Hamad MA, Stewart A, Voskuil M, 
            Vazquez-Torres A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., 
            Denver, CO 80045, USA
REFERENCE   3  (bases 1 to 4788)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4788)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2008"; volume: "3"; issue: "4"; pages: "E1976"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2008) Microbiology, UCD, 12800 E. 19th Ave., Denver, CO 
            80045, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..992
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1071..1784
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
     oriT            2111..2220
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2519..3107
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3275..3303
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             3335..4255
                     /gene="xylE"
                     /label=xylE
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"

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