Basic Vector Information
- Vector Name:
- pMN10
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3439 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Jiwaji M, Daly R, Gibriel A, Barkess G, McLean P, Yang J, Pansare K, Cumming S, McLauchlan A, Kamola PJ, Bhutta MS, West AG, West KL, Kolch W, Girolami MA, Pitt AR.
pMN10 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMN10 vector Sequence
LOCUS 40924_31160 3439 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMN10, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3439)
AUTHORS Jiwaji M, Daly R, Gibriel A, Barkess G, McLean P, Yang J, Pansare K,
Cumming S, McLauchlan A, Kamola PJ, Bhutta MS, West AG, West KL,
Kolch W, Girolami MA, Pitt AR.
TITLE Unique reporter-based sensor platforms to monitor signalling in
cells
JOURNAL PLoS ONE 7 (11), E50521 (2012)
PUBMED 23209767
REFERENCE 2 (bases 1 to 3439)
AUTHORS Jiwaji M.
TITLE The Molecular Nose
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 3439)
AUTHORS Jiwaji M.
TITLE Direct Submission
JOURNAL Submitted (17-NOV-2009) FBLS, Glasgow University, University Avenue,
Glasgow G12 8QQ, UK
REFERENCE 4 (bases 1 to 3439)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 3439)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2012"; volume: "7"; issue: "11"; pages: "E50521"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(17-NOV-2009) FBLS, Glasgow University, University Avenue, Glasgow
G12 8QQ, UK"
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3439
/mol_type="other DNA"
/organism="synthetic DNA construct"
5'UTR 137..176
/label=HSV TK 5'-UTR
/note="5' untranslated region from the herpes simplex virus
thymidine kinase gene"
misc_feature 194..316
/label=unique reporter sequence
/note="unique reporter sequence"
polyA_signal complement(350..471)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(890..1478)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1652..2509)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2510..2614)
/label=AmpR promoter
rep_origin 2641..3096
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 3227..3275
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 3289..3380
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
protein_bind 3406..3439
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
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