pMN10 vector (V004692)

LOCUS       40924_31160        3439 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMN10, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Jiwaji M, Daly R, Gibriel A, Barkess G, McLean P, Yang J, Pansare K,
            Cumming S, McLauchlan A, Kamola PJ, Bhutta MS, West AG, West KL, 
            Kolch W, Girolami MA, Pitt AR.
  TITLE     Unique reporter-based sensor platforms to monitor signalling in 
            cells
  JOURNAL   PLoS ONE 7 (11), E50521 (2012)
  PUBMED    23209767
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Jiwaji M.
  TITLE     The Molecular Nose
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3439)
  AUTHORS   Jiwaji M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2009) FBLS, Glasgow University, University Avenue,
            Glasgow G12 8QQ, UK
REFERENCE   4  (bases 1 to 3439)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3439)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "11"; pages: "E50521"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (17-NOV-2009) FBLS, Glasgow University, University Avenue, Glasgow 
            G12 8QQ, UK"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           137..176
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     misc_feature    194..316
                     /label=unique reporter sequence
                     /note="unique reporter sequence"
     polyA_signal    complement(350..471)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(890..1478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1652..2509)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2510..2614)
                     /label=AmpR promoter
     rep_origin      2641..3096
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    3227..3275
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3289..3380
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    3406..3439
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."