Basic Vector Information
- Vector Name:
- pMMRS
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10047 bp
- Type:
- Expression vector
- Replication origin:
- RSF1010 oriV
- Source/Author:
- Tao F, Liu Y, Luo Q, Su F, Xu Y, Li F, Yu B, Ma C, Xu P.
pMMRS vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMMRS vector Sequence
LOCUS 40924_31150 10047 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pMMRS, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10047)
AUTHORS Tao F, Liu Y, Luo Q, Su F, Xu Y, Li F, Yu B, Ma C, Xu P.
TITLE Novel organic solvent-responsive expression vectors for
biocatalysis: application for development of an organic
solvent-tolerant biodesulfurizing strain
JOURNAL Bioresour. Technol. 102 (20), 9380-9387 (2011)
PUBMED 21875790
REFERENCE 2 (bases 1 to 10047)
AUTHORS Tao F.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2010) School of Life Sciences and Biotechnology,
Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai,
Shanghai 200240, China
REFERENCE 3 (bases 1 to 10047)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10047)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Bioresour.
Technol."; date: "2011"; volume: "102"; issue: "20"; pages:
"9380-9387"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(31-AUG-2010) School of Life Sciences and Biotechnology, Shanghai
Jiao Tong University, 800 Dongchuan Road, Shanghai, Shanghai 200240,
China"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10047
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator 236..322
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 414..441
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 460..551
/label=AmpR promoter
CDS 552..1409
/label=AmpR
/note="beta-lactamase"
rep_origin complement(1849..2243)
/direction=LEFT
/label=RSF1010 oriV
/note="replication origin of the broad-host-range plasmid
RSF1010; requires the RSF1010 RepA/B/C proteins for
replication (Scholz et al., 1989)"
CDS complement(2270..2554)
/codon_start=1
/gene="mobC"
/product="MobC"
/label=mobC
/note="mobilization protein C"
/protein_id="AEM76696.1"
/translation="MVKGSNKAADRLAKLEEQRARINAEIQRERAREQQQERKNETRRK
VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
gene complement(2270..2554)
/gene="mobC"
/label=mobC
oriT 2585..2672
/label=RSF1010 oriT
/note="origin of transfer of the broad-host-range plasmid
RSF1010 (Scholz et al., 1989)"
CDS 3501..3914
/codon_start=1
/gene="mobB"
/product="MobB"
/label=mobB
/note="mobilization protein B"
/protein_id="AEM76698.1"
/translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTKQAS
EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
gene 3501..3914
/gene="mobB"
/label=mobB
CDS 3911..4879
/label=RSF1010 RepB
/note="replication protein B of the broad-host-range
plasmid RSF1010 (Scholz et al., 1989)"
CDS 5393..6229
/label=RSF1010 RepA
/note="replication protein A of the broad-host-range
plasmid RSF1010 (Scholz et al., 1989)"
CDS 6219..7067
/label=RSF1010 RepC
/note="replication protein C of the broad-host-range
plasmid RSF1010 (Scholz et al., 1989)"
CDS complement(7954..8595)
/codon_start=1
/gene="srpR"
/product="SrpR"
/label=srpR
/note="regulator of promoter Psrp"
/protein_id="AEM76702.1"
/translation="MARKTAAEAEETRQRIIDAALEVFVAQGVSDATLDQIARKAGVTR
GAVYWHFNGKLEVLQAVLASRQHPLELDFTPDLGIERSWEAVVVAMLDAVHSPQSKQFS
EILIYQGLDESGLIHNRMVQASDRFLQYIHQVLRHAVTQGELPINLDLQTSIGVFKGLI
TGLLYEGLRSKDQQAQIIKVALGSFWALLREPPRFLLCEEAQIKQVKSFE"
gene complement(7954..8595)
/gene="srpR"
/label=srpR
CDS complement(8601..9380)
/codon_start=1
/gene="srpS"
/product="SrpS"
/label=srpS
/note="regulator of promoter Psrp"
/protein_id="AEM76703.1"
/translation="MNQSDENVGKAGGIQVIARAASIMRALGSHPHGLSLAAIAQLVGL
PRSTVQRIINALEEEFLVEALGPAGGFRLGPALGQLINQAQSDILSLVKPYLRSLAEEL
EESVCLASLAGDKIYVLDRIVSERELRVVFPIGINVPAAATAAGKVLLAALPDETLQAA
LGEQLPVFTSNTLRRKALVKQLSEVRQSGFASDLDEHIDGVCSFATLLDTYLGYYSLAV
VMPSSRASKQSDLIKKALLQSKQNIERAIGRASKKAP"
gene complement(8601..9380)
/gene="srpS"
/label=srpS
promoter complement(9613..9690)
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
regulatory 9954..10003
/gene="Psrp"
/regulatory_class="promoter"
gene 9954..10003
/gene="Psrp"
/label=Psrp
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