Basic Vector Information
- Vector Name:
- pMM21
- Antibiotic Resistance:
- Gentamycin
- Length:
- 4849 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Dean C, Barkan D, Bermingham A, Blais J, Casey F, Casarez A, Colvin R, Fuller J, Jones A, Li C, Lopez S, Metzger L, Prathapam R, Rasper D, Reck F, Ruzin A, Shaul J, Shen X, Simmons R, Skewes-Cox P, Ta
- Promoter:
- Pc
pMM21 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMM21 vector Sequence
LOCUS 40924_31115 4849 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMM21, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4849)
AUTHORS Dean C, Barkan D, Bermingham A, Blais J, Casey F, Casarez A, Colvin
R, Fuller J, Jones A, Li C, Lopez S, Metzger L, Prathapam R, Rasper
D, Reck F, Ruzin A, Shaul J, Shen X, Simmons R, Skewes-Cox P,
Tamrakar P, Uehara T.
TITLE The monobactam LYS228: mode of action and mechanisms decreasing in
vitro susceptibility of Escherichia coli and Klebsiella pneumoniae
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4849)
AUTHORS Wei J-R., Mostafavi M, Takeoka K.
TITLE Direct Submission
JOURNAL Submitted (27-JUL-2018) Infectious Diseases, Bacteriology, Novartis
Institutes for Biomedical Research, 5300 Chiron way, Emeryville, CA
94608, USA
REFERENCE 3 (bases 1 to 4849)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4849)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-JUL-2018) Infectious Diseases, Bacteriology, Novartis Institutes
for Biomedical Research, 5300 Chiron way, Emeryville, CA 94608, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4849
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(83..1501)
/label=SacB
/note="secreted levansucrase that renders bacterial growth
sensitive to sucrose"
promoter 1738..1766
/label=Pc promoter
/note="class 1 integron promoter"
CDS 1955..2485
/label=GmR
/note="gentamycin acetyltransferase"
promoter 2794..2822
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
misc_RNA 2828..2847
/product="gRNA variable region"
misc_RNA 2848..2923
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
terminator 2945..2979
/label=lambda t0 terminator
/note="minimal transcription terminator from phage lambda
(Scholtissek and Grosse, 1987)"
rep_origin 3019..3474
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 3585..4173
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(4359..4499)
/label=bom
/note="basis of mobility region from pBR322"
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