pML1381 vector (V004706)

LOCUS       40924_31085        5540 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pML1381, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE.
  TITLE     An enzyme-coupled biosensor enables (S)-reticuline production in 
            yeast from glucose
  JOURNAL   Nat. Chem. Biol. 11 (7), 465-471 (2015)
  PUBMED    25984720
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJJ., Dueber 
            JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2015) Department of Bioengineering, University of 
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA 
            94704, USA
REFERENCE   3  (bases 1 to 5540)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5540)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. 
            Biol."; date: "2015"; volume: "11"; issue: "7"; pages: "465-471"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2015) Department of Bioengineering, University of 
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA 
            94704, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..524
                     /label=similar to LEU2 5'
                     /note="similar to LEU2 5'"
     misc_feature    687..692
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     misc_feature    694..697
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     misc_feature    complement(764..767)
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     misc_feature    complement(769..774)
                     /label=BsmBI recognition site
                     /note="BsmBI recognition site"
     promoter        1177..1584
                     /label=LEU2 promoter
     CDS             1585..2676
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     regulatory      2680..3156
                     /regulatory_class="terminator"
     misc_feature    3161..3670
                     /label=similar to LEU2 3'
                     /note="similar to LEU2 3'"
     regulatory      complement(3683..3779)
                     /regulatory_class="terminator"
     CDS             complement(3780..4637)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4638..4742)
                     /label=AmpR promoter
     rep_origin      4845..5433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"