pMK502-TEV vector (V004709)

LOCUS       V004709                 8330 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004709
VERSION     V004709
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Guo D, Hazbun TR, Xu XJ, Ng SL, Fields S, Kuo MH.
  TITLE     A tethered catalysis, two-hybrid system to identify protein-protein
            interactions requiring post-translational modifications
  JOURNAL   Nat. Biotechnol. 22 (7), 888-892 (2004)
   PUBMED   15208639
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Kuo M-H., Xu X-J., Ng S-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2004) Biochem. Mol. Biol., Michigan State
            University, 309 BCH Building, East Lansing, MI 48824, USA
REFERENCE   3  (bases 1 to 8330)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8330)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2004"; volume: "22"; issue: "7"; pages:
            "888-892"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JUN-2004) Biochem. Mol. Biol., Michigan State University, 309
            BCH Building, East Lansing, MI 48824, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             434..874
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             932..952
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             1040..1957
                     /gene="KIN28"
                     /label="Serine/threonine-protein kinase KIN28"
                     /note="Serine/threonine-protein kinase KIN28 from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P06242"
     CDS             1991..2017
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2021..2047
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2054..2080
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     terminator      2519..2706
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    complement(3538..4700)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     promoter        complement(5214..5315)
                     /label="TRP1 promoter"
     promoter        5421..5525
                     /label="AmpR promoter"
     CDS             5526..6383
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6557..7145
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"