Basic Vector Information
- Vector Name:
- pMK502-TEV
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8330 bp
- Type:
- Yeast two-hybrid vector
- Replication origin:
- ori
- Source/Author:
- Guo D, Hazbun TR, Xu XJ, Ng SL, Fields S, Kuo MH.
- Promoter:
- TRP1
pMK502-TEV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMK502-TEV vector Sequence
LOCUS 40924_31020 8330 bp DNA circular SYN 18-DEC-2018
DEFINITION Yeast two-hybrid vector pMK502-TEV, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8330)
AUTHORS Guo D, Hazbun TR, Xu XJ, Ng SL, Fields S, Kuo MH.
TITLE A tethered catalysis, two-hybrid system to identify protein-protein
interactions requiring post-translational modifications
JOURNAL Nat. Biotechnol. 22 (7), 888-892 (2004)
PUBMED 15208639
REFERENCE 2 (bases 1 to 8330)
AUTHORS Kuo M-H., Xu X-J., Ng S-L.
TITLE Direct Submission
JOURNAL Submitted (08-JUN-2004) Biochem. Mol. Biol., Michigan State
University, 309 BCH Building, East Lansing, MI 48824, USA
REFERENCE 3 (bases 1 to 8330)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8330)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
Biotechnol."; date: "2004"; volume: "22"; issue: "7"; pages:
"888-892"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(08-JUN-2004) Biochem. Mol. Biol., Michigan State University, 309
BCH Building, East Lansing, MI 48824, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8330
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 434..874
/label=GAL4 DNA binding domain
/note="DNA binding domain of the GAL4 transcriptional
activator"
CDS 932..952
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
CDS 1040..1957
/gene="KIN28"
/label=KIN28
/note="Serine/threonine-protein kinase KIN28 from
Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
Accession#: P06242"
CDS 1991..2017
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS 2021..2047
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS 2054..2080
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
terminator 2519..2706
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
misc_feature complement(3538..4700)
/label=CEN/ARS
/note="S. cerevisiae CEN4 centromere fused to the
autonomously replicating sequence ARS1/ARS416"
promoter complement(5214..5315)
/label=TRP1 promoter
promoter 5421..5525
/label=AmpR promoter
CDS 5526..6383
/label=AmpR
/note="beta-lactamase"
rep_origin 6557..7145
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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