Basic Vector Information
- Vector Name:
- pMK33-CTAP(SG)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8793 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Kyriakakis P, Tipping M, Abed L, Veraksa A.
- Promoter:
- MT
pMK33-CTAP(SG) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMK33-CTAP(SG) vector Sequence
LOCUS 40924_30980 8793 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMK33-CTAP(SG), complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8793)
AUTHORS Kyriakakis P, Tipping M, Abed L, Veraksa A.
TITLE Tandem affinity purification in Drosophila: The advantages of the
GS-TAP system
JOURNAL Fly (Austin) 2 (4) (2008) In press
PUBMED 18719405
REFERENCE 2 (bases 1 to 8793)
AUTHORS Veraksa A, Kyriakakis P, Tipping M, Abed L.
TITLE Direct Submission
JOURNAL Submitted (20-APR-2008) Biology Department, University of
Massachusetts Boston, 100 Morrissey Blvd., Boston, MA 02125, USA
REFERENCE 3 (bases 1 to 8793)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8793)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Fly
(Austin) 2 (4) (2008) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(20-APR-2008) Biology Department, University of Massachusetts
Boston, 100 Morrissey Blvd., Boston, MA 02125, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8793
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(865..1453)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1627..2484)
/label=AmpR
/note="beta-lactamase"
promoter complement(2485..2589)
/label=AmpR promoter
promoter 4071..4350
/label=copia promoter
/note="strong promoter from the Drosophila transposable
element copia (Sinclair et al., 1986)"
CDS 4396..5418
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
intron 5784..5849
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 5979..5999
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 6424..6558
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 6559..6985
/label=MT promoter
/note="Drosophila metallothionein promoter"
misc_feature 6992..7021
/note="polylinker; unique cloning sites for XhoI, BamHI,
EcoRV, KpnI, SpeI"
CDS 7025..7138
/label=SBP
/note="streptavidin-binding peptide"
CDS 7145..7165
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
CDS 7172..7192
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
This page is informational only.