pMK2016 vector (V004720)

LOCUS       V004720                 6981 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004720
VERSION     V004720
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   House BL, Mortimer MW, Kahn ML.
  TITLE     New recombination methods for Sinorhizobium meliloti genetics
  JOURNAL   Appl. Environ. Microbiol. 70 (5), 2806-2815 (2004)
   PUBMED   15128536
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   House BL, Mortimer MW, Kahn ML.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2003) School of Molecular Biosciences, Washington
            State University, Abelson Hall Room 406A, Pullman, WA 99164, USA
REFERENCE   3  (bases 1 to 6981)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6981)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2004"; volume: "70"; issue: "5"; pages:
            "2806-2815"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-SEP-2003) School of Molecular Biosciences, Washington State
            University, Abelson Hall Room 406A, Pullman, WA 99164, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      605..1195
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1615..1803)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             1843..2187
                     /gene="mbeC"
                     /label="Mobilization protein MbeC"
                     /note="Mobilization protein MbeC from Escherichia coli.
                     Accession#: P13657"
     primer_bind     3507..3523
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(3526..3582)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     protein_bind    complement(3599..3646)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3687..3806
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3836..3866
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             3920..4576
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             4921..5223
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(5267..5391)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    5432..5479
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    5496..5552
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(5803..6591)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"