Basic Vector Information
- Vector Name:
- pMJ22
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4509 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Martinez Jimenez V, Castano Navarro I.
- Promoter:
- TEF1
pMJ22 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMJ22 vector Sequence
LOCUS 40924_30930 4509 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMJ22, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4509)
AUTHORS Martinez Jimenez V, Castano Navarro I.
TITLE Expression vectors for C-terminal fusions with fluorescent proteins
and epitope tags in C. glabrata
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4509)
AUTHORS Martinez Jimenez V, Castano Navarro I.
TITLE Direct Submission
JOURNAL Submitted (05-DEC-2014) Division de Biologia Molecular, Instituto
Potosino de Investigacion Cientifica y Tecnologica, Camino a la
Presa San Jose 2055 Col. Lomas 4 seccion, San Luis Potosi 78216,
Mexico
REFERENCE 3 (bases 1 to 4509)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4509)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(05-DEC-2014) Division de Biologia Molecular, Instituto Potosino de
Investigacion Cientifica y Tecnologica, Camino a la Presa San Jose
2055 Col. Lomas 4 seccion, San Luis Potosi 78216, Mexico"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4509
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 347..747
/label=TEF1 promoter
/note="promoter for EF-1-alpha"
CDS 767..1330
/codon_start=1
/label=NrsR
/note="nourseothricin acetyltransferase"
/translation="TTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFTL
REVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRRL
TVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRMGFTLC
GLDTALYDGTASDGEQALYMSMPCP"
terminator 1340..1587
/label=CYC1 terminator
/note="transcription terminator for CYC1"
misc_feature complement(1592..1699)
/label=MCS
/note="pBluescript multiple cloning site"
promoter complement(1712..1730)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(1751..1767)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(1775..1791)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1799..1829)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(1844..1865)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2153..2741)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2915..3772)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3773..3844)
/label=AmpR promoter
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